NOVEL BIOACTIVE FLAVONOID 5-HYDROXY-2-(8-HYDROXY 4-OXO-4HCHROMEN-2-YL)-4-OXO-3, 4-DIHYDRO-2H-1-BENZOPYRAN-7-YL N-[(2-METHYLPROPOXY) CARBONYL] CARBAMATE FROM KALANCHOE PINNATA

Kalanchoe Pinnata is a conspicuous medicinal plant and finds wide applications in pharmaceutical industries. Phytochemical studies approve the mode of action of plant extract as bioactive agent. The foremost constituents accountable for biological activity are polyphenols are important class of naturally occurring antioxidants, having innumerable biological activities such as anticancer, antifungal, antibacterial, antiviral and anti-cholesterol. The present study was focussed on extraction, identification and characterization of flavonoids present in leaves of Kalanchoe pinnata. The purified fractions of the methanol extract were quantised by preparative TLC and RP-HPLC to achieve maximum purity. The isolated purified compounds were studied for spectral characterisation like IR, H1- NMR, C13-NMR and MS. On the basis of chemical constitution and spectral analysis, the structure was elucidated as a 5-hydroxy-2-(8-hydroxy 4-oxo-4Hchromen-2-yl)-4-oxo-3, 4-dihydro-2H-1-benzopyran-7-yl N-[(2-methylpropoxy) carbonyl] carbamate. Combined bioactivities to be evaluated along with other known compounds like gallic acid and quercetin.