FRET-based mesoporous organosilica nanoplatforms for <i>in vitro</i> and <i>in vivo</i> anticancer two-photon photodynamic therapy
We report the synthesis of multifunctional periodic mesoporous organosilica nanoparticles (PMO NPs) with substantial two-photon absorption properties and targeting capability for two-photon excitation fluorescence (TPEF) and photodynamic therapy (TPE-PDT). Prepared using an adapted sol-gel synthesis, the nanoplatforms integrated two silylated chromophores in their three-dimensional matrix to maximize non-radiative Förster resonance energy transfer from a high two-photon absorption fluorophore donor to a porphyrin derivative acceptor, leading to an enhanced generation of reactive oxygen species. Combinations of biodegradable and non-biodegradable bis(triethoxysilyl)alkoxysilanes were employed for the synthesis of the NPs, and the corresponding photophysical studies revealed high efficiency levels of FRET. Next, the cellular uptake and toxicities of pristine and functionalized NPs were evaluated on breast cancer cell lines upon TPEF and TPE-PDT. Notably, the use of TPE-PDT treatment led to high levels of phototoxicity on MCF-7 and MDA-MB-231 cancer cells with substantial effects when compared to one-photon excitation (OPE)-PDT treatment. Preliminary <i>in vivo</i> data on selective and biodegradable NPs showed a significant phototoxicity towards MDA-MB-231 on zebrafish xenograft embryos, making these advanced nanoplatforms promising candidates for future TPE-PDT-based cancer treatments.
Authors
- D. Durand (ORCID: https://orcid.org/0000-0001-8381-8999)
- Alexandre Barras (ORCID: https://orcid.org/0000-0003-2821-7079)
- Frédéric Paul (ORCID: https://orcid.org/0000-0002-8256-0129)
- Jean‐Olivier Durand (ORCID: https://orcid.org/0000-0003-4606-2576)
- Clarence Charnay (ORCID: https://orcid.org/0000-0002-8796-3701)
- Nicolas Richy
- Nadir Bettache (ORCID: https://orcid.org/0000-0001-5426-7024)
- Erwan Oliviero (ORCID: https://orcid.org/0000-0002-7828-9137)
- Olivier Mongin (ORCID: https://orcid.org/0000-0002-7743-9623)
- Grégory Taupier (ORCID: https://orcid.org/0000-0002-5965-4979)
- Lamiaa M. A. Ali (ORCID: https://orcid.org/0000-0003-1176-5335)
- Magali Gary‐Bobo (ORCID: https://orcid.org/0000-0001-9641-212X)
- Nicolas Bondon (ORCID: https://orcid.org/0000-0002-1943-8024)
- Christophe Nguyen (ORCID: https://orcid.org/0000-0003-4539-1677)
- Yann Molard (ORCID: https://orcid.org/0000-0002-6295-0883)
- Rabah Boukherroub (ORCID: https://orcid.org/0000-0002-9795-9888)
- C. Charlot
- Sabine Szunerits (ORCID: https://orcid.org/0000-0002-1567-4943)
Institutions
- Centre National de la Recherche Scientifique (FR)
- Université de Montpellier (FR)
- Université de Lille (FR)
- Institut des Sciences Chimiques de Rennes (FR)
- Institut d'Electronique, de Microélectronique et de Nanotechnologie (FR)
- Institut des Biomolécules Max Mousseron (FR)
- Université de Rennes (FR)
- Université Polytechnique Hauts-de-France (FR)
- Alexandria University (EG)
Publication Details
- Journal
- Journal of Materials Chemistry B
- Published
- 2024-12-24
- DOI
- https://doi.org/10.1039/d4tb02103g
- Citations
- 2
- Primary Topic
- Nanoplatforms for cancer theranostics
- Type
- article
- Field-Weighted Citation Impact
- 0.37
Funders
- Agence Nationale de la Recherche