Screening of salt tolerance indexes in embryogenic and non-embryogenic callus of grape

Salt stress seriously affects grape yield and quality and has become one of the major abiotic stresses that constrain the development of the grape industry. In the actual research process, it is difficult and time costing to obtain transgenic grape seedlings through genetic transformation system. Therefore, the establishment of a rapid identification system of salt tolerance in grapes using callus as materials is necessary to identify the function of genes in salt stress response. In the present study, different concentrations of NaCl were applied to embryogenic and non-embryogenic callus tissues, and salt tolerance indexes were measured. The results were analyzed to determine the best indexes to characterize the salt tolerance of different grapevine callus, which would lay the foundation for the rapid identification of salt tolerance genes in grapes. The growth status was observed after 28 days and parameters such as growth (in dishes), MDA content, free proline content and relative electrical conductivity were determined. It was ultimately found that electrical conductivity increased more in merlot and shiraz than in other salt tolerance indicators and was the most sensitive to changes in salt stress, so it was determined that electrical conductivity could be used tomonitor changes in salt tolerance in grapevine callus tissues.