Bioinspired design of DNA in aqueous ionic liquid media for sustainable packaging of horseradish peroxidase under biotic stress
We show that a combination of DNA and ionic liquid significantly increases the stability and activity of HRP and achieves a 4.8-fold higher peroxidase activity than PBS buffer. Also, HRP retains 84% of its activity in IL+DNA compared to 24% in PBS against trypsin digestion. Molecular modeling and spectroscopic studies reveal a protective microenvironment.
Authors
- Aaftaab Sethi (ORCID: https://orcid.org/0000-0003-2471-9813)
- Gregory Franklin (ORCID: https://orcid.org/0000-0002-6745-3528)
- Diksha Dhiman (ORCID: https://orcid.org/0000-0002-0154-2325)
- Sagar Biswas (ORCID: https://orcid.org/0000-0001-5797-2853)
- Rakesh Sinha (ORCID: https://orcid.org/0000-0003-3090-058X)
- Dibyendu Mondal (ORCID: https://orcid.org/0000-0002-1715-5514)
Institutions
- Jain University (IN)
- Institute of Plant Genetics, Polish Academy of Sciences (PL)
- Polish Academy of Sciences (PL)
Publication Details
- Journal
- Chemical Communications
- Published
- 2024-12-23
- DOI
- https://doi.org/10.1039/d4cc05803h
- Primary Topic
- Nanocomposite Films for Food Packaging
- Type
- article
- Field-Weighted Citation Impact
- 0.00