Pseudomonas fluorescens, Bacillus megaterium, and Pseudomonas putida in the restoration of technogenically disturbed territories of the Kuzbass

The Kuznetsk Basin (Kuzbass) is the largest Russian region specializing in mining. Most mining operations in the region are carried out by the open method, which is the safest and most effective, but contributes to the formation of a significant amount of technogenically disturbed land. To form a fertile soil layer and vegetation cover, it is rational to use microorganisms together with plants. Microorganisms are known to be able to intensify plant growth by improving their nutrition, synthesis of growth-stimulating substances, and protection from phytopathogens. The greatest growth-stimulating properties were noted for representatives of the genera Pseudomonas and Bacillus . In turn, some plants, in particular the granary ( Agropyron cristatum L.), form stable herbage and participate in the formation of the surface horizon of soils (sod). The purpose of the work was to study the prospects for the joint use of Pseudomonas fluorescens B-4252, Bacillus megaterium B-3778, Pseudomonas putida B-2950, and granary to increase fertility and form a stable vegetation cover in the technogenically disturbed territories of the Kuznetsk Basin. It has been established that the studied strains are capable of producing prussic acid, phytohormones, as well as phosphorus solubilization and nitrogen fixation. These strains and consortia based thereon improve the growth of the granary in laboratory conditions. Seed treatment with the above microorganisms contributes to an increase in germination and the growth rate of the granary. E.g., the germination of seeds during processing by consortium No. 2 was 94%, which was 12% more compared to the control (without treatment). The average length of the sprouts in this treatment option was 56 mm, which was more than 17 mm for the control. Due to the described qualities, the consortium could become an effective agent to accelerate the restoration of the Kuzbass technozems.