Exploiting gasdermin-mediated pyroptosis for enhanced antimicrobial activity of phage endolysin against <i>Pseudomonas aeruginosa</i>
Recombinant GSDMD<sub>Nterm</sub> protein was able to efficiently permeabilize <i>P. aeruginosa</i> outer membranes and increase endolysin activity against bacteria, producing either long LPS O-chains or lack them entirely. The obtained results suggest the limited possibility of using the natural process of pyroptosis occurring in monocytic cells to enhance the bactericidal effect of recombinant phage endolysins against Gram-negative bacteria infection.
Authors
- Karol Ciepluch (ORCID: https://orcid.org/0000-0003-3180-7715)
- Dorota Kuc‐Ciepluch (ORCID: https://orcid.org/0000-0001-8333-3326)
- Michał Arabski (ORCID: https://orcid.org/0000-0002-9712-9286)
- Artur Kowalik (ORCID: https://orcid.org/0000-0002-3718-999X)
- Daria Augustyniak (ORCID: https://orcid.org/0000-0002-0547-3937)
- Zuzanna Drulis‐Kawa (ORCID: https://orcid.org/0000-0002-4733-4660)
- Grzegorz Guła (ORCID: https://orcid.org/0000-0003-1320-884X)
- Barbara Maciejewska (ORCID: https://orcid.org/0000-0002-4876-8743)
- Ewelina Jop
Institutions
- College of the Holy Cross (US)
- Jan Kochanowski University (PL)
- University of Wrocław (PL)
Publication Details
- Journal
- mSystems
- Published
- 2024-12-23
- DOI
- https://doi.org/10.1128/msystems.01106-24
- Citations
- 2
- Primary Topic
- Inflammasome and immune disorders
- Type
- article
- Field-Weighted Citation Impact
- 0.47
Funders
- Narodowe Centrum Nauki