Quantitative analysis of Streptococcus mutans, Bifidobacterium, and Scardovia Wiggsiae in occlusal biofilm and their association with Visible Occlusal Plaque Index (VOPI) and International Caries Detection and Assessment System (ICDAS)

Quantities of total bacteria (p < 0.001), S. mutans (p = 0.02) and ICDAS scores (p < 0.001) and VOPI scores were positively correlated. Quantities of ratio of S. mutans to total bacteria (p = 0.003) and ratio of Bifidobacterium to total bacteria (p < 0.001) and VOPI scores and ICDAS scores (p < 0.001) were negatively correlated.