Performance evaluation of nine reference centers and comparison of DNA extraction protocols for effective surveillance of Leishmania-infected Phlebotomine sand flies: Basis for technical recommendations
This study emphasizes the importance of standardized DNA extraction protocols and continuous quality assurance for accurate Leishmania DNA detection. The results highlight the superior performance of certain extraction kits and the need for ongoing technical training, essential for reliable leishmaniasis surveillance, particularly in field settings with low infection densities.
Authors
- J. Risueño (ORCID: https://orcid.org/0000-0001-5006-7738)
- Trentina Di Muccio (ORCID: https://orcid.org/0000-0001-6452-8902)
- Mesut Mungan
- Clara Muñoz (ORCID: https://orcid.org/0000-0001-9847-8616)
- Oscar David Kirstein (ORCID: https://orcid.org/0000-0003-4765-7483)
- Jean‐Pierre Gangneux (ORCID: https://orcid.org/0000-0002-4974-5607)
- Kardelen Yetişmiş (ORCID: https://orcid.org/0000-0001-7111-5807)
- Jorian Prudhomme (ORCID: https://orcid.org/0000-0002-3672-2692)
- Carla Maia (ORCID: https://orcid.org/0000-0002-2545-7686)
- Gioia Bongiorno
- E. Berriatua (ORCID: https://orcid.org/0000-0002-0721-8091)
- Ceylan Polat (ORCID: https://orcid.org/0000-0003-1511-4177)
- Julia Walochnik (ORCID: https://orcid.org/0000-0003-0356-2853)
- Muhammed Nalçacı (ORCID: https://orcid.org/0000-0002-9265-2887)
- Eleonora Fiorentino
- Ricardo Parreira (ORCID: https://orcid.org/0000-0002-4832-9898)
- Maya Davidovich-Cohen
- Bülent Alten (ORCID: https://orcid.org/0000-0002-9711-4113)
- Liora Studentsky (ORCID: https://orcid.org/0000-0002-1469-4301)
- Gamze Varol
- José Manuel Cristóvão (ORCID: https://orcid.org/0000-0003-3861-9346)
- Özge Erişöz Kasap (ORCID: https://orcid.org/0000-0002-5673-4180)
- Seray Ozensoy Töz (ORCID: https://orcid.org/0000-0001-5957-8665)
- Edwin Kniha (ORCID: https://orcid.org/0000-0002-6875-7056)
- Umut Berberoglu
- Yusuf Özbel (ORCID: https://orcid.org/0000-0001-8335-1997)
- Aymeric Delabarre
- Katharina Platzgummer
- Gizem Oguz Kaskan
Institutions
- Ministry of Health (TR)
- Inserm (FR)
- École des Hautes Études en Santé Publique (FR)
- Istituto Superiore di Sanità (IT)
- Israel Ministry of Health (IL)
- Ege University (TR)
- Instituto de Investigación en Recursos Cinegéticos (ES)
- Institut de Recherche en Santé, Environnement et Travail (FR)
- Université de Rennes (FR)
- Hacettepe University (TR)
- Medical University of Vienna (AT)
- Universidad de Murcia (ES)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2024-12-23
- DOI
- https://doi.org/10.1371/journal.pntd.0012543
- Citations
- 7
- Primary Topic
- Research on Leishmaniasis Studies
- Type
- article
- Field-Weighted Citation Impact
- 3.80
Funders
- Univerzita Karlova v Praze
- UK Research and Innovation
- European Commission
- Institut National de la Santé et de la Recherche Médicale