A Proteomic Examination of Plasma Extracellular Vesicles Across Colorectal Cancer Stages Uncovers Biological Insights That Potentially Improve Prognosis
By employing stringent proteomic analysis and a robust SWATH-MS approach, we identified dysregulated EV proteins that potentially indicate early-stage CRC and predict recurrence risk, including proteins involved in metabolism, cytoskeletal remodelling, and immune response. While our findings underline discrepancies with other studies due to differing isolation and stringency parameters, they provide valuable insights into the complexity of the EV proteome, emphasising the need for standardised protocols and larger, well-controlled studies to validate potential biomarkers.
Authors
- Shivani Krishnamurthy (ORCID: https://orcid.org/0000-0002-7418-4338)
- Joo‐Shik Shin (ORCID: https://orcid.org/0000-0001-7840-3625)
- David Cantor (ORCID: https://orcid.org/0000-0001-7210-7227)
- Benjamin Heng (ORCID: https://orcid.org/0000-0002-0247-2920)
- Seong Beom Ahn (ORCID: https://orcid.org/0000-0001-5907-3544)
- Mark S. Baker (ORCID: https://orcid.org/0000-0001-5858-4035)
- Michael J. Solomon (ORCID: https://orcid.org/0000-0002-5602-6045)
- Ardeshir Amirkhani
- Gilles J. Guillemin (ORCID: https://orcid.org/0000-0001-8105-4470)
- Peter Jun Myung Lee
- Abidali Mohamedali (ORCID: https://orcid.org/0000-0001-5627-4105)
Institutions
- The University of Sydney (AU)
- IPB University (ID)
- Royal Prince Alfred Hospital (AU)
- AGRF Ltd (AU)
- Macquarie University (AU)
Publication Details
- Journal
- Cancers
- Published
- 2024-12-21
- DOI
- https://doi.org/10.3390/cancers16244259
- Citations
- 3
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- Macquarie University
- Cancer Council NSW