The CP123L protein of African swine fever virus is a membrane-associated, palmitoylated protein required for viral replication
African swine fever (ASF) poses a significant threat to the global pig industry. The causative agent of ASF is African swine fever virus (ASFV), which encodes more than 165 proteins. Protein palmitoylation, a common posttranslational lipid modification, can modulate viral infection. To date, the ASFV proteins that undergo palmitoylation and their impacts on viral replication remain elusive. In this study, the CP123L protein (pCP123L) of ASFV was identified as a palmitoylated protein, and the cysteine residue at position 18 of pCP123L is responsible for its palmitoylation. Notably, our findings demonstrate that palmitoylation plays significant roles in ASFV protein functions and facilitates viral replication.
Authors
- Hua‐Ji Qiu (ORCID: https://orcid.org/0000-0003-4880-5687)
- Fengwei Jiang (ORCID: https://orcid.org/0000-0002-2353-1897)
- Hongxia Wu (ORCID: https://orcid.org/0000-0002-7317-7670)
- Xiaoke Yang
- Huanjie Zhai
- Xiangyu Guan (ORCID: https://orcid.org/0000-0003-2802-9406)
- Lian‐Feng Li (ORCID: https://orcid.org/0000-0001-9653-5533)
- Su Li (ORCID: https://orcid.org/0000-0002-5845-4617)
- Yongfeng Li
- Qinghe Hou
- Yuan Sun (ORCID: https://orcid.org/0009-0009-0518-9336)
- Yuzi Luo
- Yuxuan Gao
- Tao Wang (ORCID: https://orcid.org/0009-0003-1825-0163)
Institutions
- Harbin Veterinary Research Institute (CN)
Publication Details
- Journal
- Journal of Virology
- Published
- 2024-12-23
- DOI
- https://doi.org/10.1128/jvi.01445-24
- Citations
- 2
- Primary Topic
- Animal Disease Management and Epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 1.14
Funders
- National Natural Science Foundation of China
- National Key Research and Development Program of China