ANTIMICROBIAL ASSAY OF ESSENTIAL OIL FROM Eucalyptus globulus LEAF

Investigating the antibacterial test of essential oil derived from Eucalyptus globulus leaves against clinically significant microbial strains is the goal of this investigation. Salmonella typhi, Pseudomonas aeruginosa, and Escherichia coli are the isolates of Gram-negative bacteria, whereas Staphylococcus aureus is an isolate of Gram-positive bacteria. In the current study, essential oil was extracted using microwave-assisted hydrodistillation, and the oil's antibacterial properties were assessed. The physical method was used to determine the oil's color, odor, and solubility. The essential oil of fresh Eucalyptus globulus leaves exhibits zone of inhibition against three of the studied species Escherichia coli, Staphylococcus aureus and Salmonella typhi at all concentrations of oil per disc but no action against Salmonella typhi at 1.43 mL/disc. At all concentrations, the essential oil did not, however, exhibit any anti-Pseudomonas aeruginosa activity. With a zone of inhibition of 17.7 mm and a concentration of 5.72 mL/disc, the oil was most effective against Staphylococcus aureus. As concentration drops, the zone of inhibition shrinks as well; at 2.86 mL/disc and 1.43 mL/disc, it is 14.7 mm and 9.7 mm, respectively. The essential oil of dried Eucalyptus globulus leaves exhibits no action against Salmonella typhi at 1.43 mL/disc, but it does exhibit a zone of inhibition against three of the examined organisms: Salmonella typhi, Staphylococcus aureus, and Escherichia coli, at all oil concentrations per disc. At all concentrations, the essential oil did not, however, exhibit any anti-Pseudomonas aeruginosa activity. With a zone of inhibition of 13.3 mm and a concentration of 5.72 mL/disc, the oil was most effective against Staphylococcus aureus. As concentration drops, the zone of inhibition shrinks as well; at 2.86 mL/disc and 1.43 mL/disc, it is 11.1 mm and 8.3 mm, respectively. It is advised that the essential oil's effectiveness be evaluated against fungus in order to investigate the best combinations and uses to maximize its medicinal potential. For direct use, in vivo assay is advised.