Characterization of 3,3′-iminodipropionitrile (IDPN) damaged utricle transcriptome in the adult mouse utricle
Utricle is an important vestibular sensory organ for maintaining balance. 3,3'-iminodipropionitrile (IDPN), a prototype nitrile toxin, has been reported to be neurotoxic and vestibulotoxic, and can be used to establish an <i>in vivo</i> damage model of vestibular dysfunction. However, the mechanism of utricular HCs damage caused by IDPN is unclear. Here, we first studied mice balance behavior and HCs damage in IDPN utricle damage model, and found that IDPN injection <i>in vivo</i> can cause vestibular dysfunction and HCs damage, which is more pronounced than neomycin damage model. Then we used RNA-seq to characterize the transcriptome of IDPN damaged utricle in detail to identify genes and pathways that play roles in this process. We found 1,165 upregulated genes and 1,043 downregulated genes in IDPN damaged utricles, and identified that NF-κB pathway and TNF pathway may play important roles in IDPN damage model. Our study provides details of transcriptome of IDPN utricle damage model for further study of vestibular dysfunction.
Authors
- Xia Sheng (ORCID: https://orcid.org/0000-0002-8742-7343)
- Pei Jiang (ORCID: https://orcid.org/0000-0002-8360-7427)
- Tong Wei (ORCID: https://orcid.org/0000-0002-2692-7192)
- Jingyuan Huang (ORCID: https://orcid.org/0000-0003-2273-9917)
- Zixuan Ye (ORCID: https://orcid.org/0000-0002-2209-5034)
- Mingchen Dai
- Renjie Chai (ORCID: https://orcid.org/0000-0002-3885-543X)
- Yintao Wang (ORCID: https://orcid.org/0000-0001-6360-4686)
- Hairong Xiao
- Yanqin Lin (ORCID: https://orcid.org/0000-0003-0596-0740)
- Xiangyu Ma (ORCID: https://orcid.org/0000-0002-5657-1991)
- Xujun Tang (ORCID: https://orcid.org/0009-0007-9584-1919)
- Shasha Zhang (ORCID: https://orcid.org/0009-0004-7473-2922)
- Mengyao Tian
Institutions
- University of Electronic Science and Technology of China (CN)
- Capital Medical University (CN)
- Nantong University (CN)
- Hainan University (CN)
- Zhongda Hospital Southeast University (CN)
- Center for Excellence in Molecular Cell Science (CN)
- State Key Laboratory of Digital Medical Engineering (CN)
- Huazhong University of Science and Technology (CN)
- Southeast University (CN)
Publication Details
- Journal
- Frontiers in Molecular Neuroscience
- Published
- 2024-12-23
- DOI
- https://doi.org/10.3389/fnmol.2024.1487364
- Citations
- 1
- Primary Topic
- Hearing, Cochlea, Tinnitus, Genetics
- Type
- article
- Field-Weighted Citation Impact
- 0.36
Funders
- National Natural Science Foundation of China
- Fudan University
- Department of Science and Technology of Sichuan Province
- State Key Laboratory of Genetic Engineering
- Basic and Applied Basic Research Foundation of Guangdong Province