A Simplified Model of Adenine-Induced Chronic Kidney Disease Using SKH1 Mice
Commonly used adenine-induced chronic kidney disease (CKD) murine models often employ C57BL/6 mice; however, this strain has inherent limitations due to its natural resistance to developing key pathological features of CKD, such as tubulointerstitial fibrosis and inflammation. There have been attempts to overcome these barriers by using multiple concentrations of adenine-supplemented diets or by performing prolonged experiments up to 20 weeks. Here, we demonstrate that SKH1 Elite mice develop clinically relevant CKD phenotypes (e.g., polyuria, proteinuria, inflammation, and renal fibrosis) over the course of only 6 weeks of low-dose (0.15%) adenine supplementation. As a docile, immunocompetent, and hairless strain, SKH1 Elite mice offer several logistical advantages over C57BL/6 mice, including ease of handling and the ability to study dermal conditions, which are often secondary to CKD.
Authors
- Apurva Lad (ORCID: https://orcid.org/0000-0001-6073-7432)
- Benjamin W. French (ORCID: https://orcid.org/0000-0002-7861-6740)
- Steven T. Haller (ORCID: https://orcid.org/0000-0002-6919-6342)
- David J. Kennedy (ORCID: https://orcid.org/0000-0001-5265-0142)
- Bivek Timalsina (ORCID: https://orcid.org/0000-0003-0734-0780)
- Amira F. Gohara (ORCID: https://orcid.org/0000-0001-7068-2244)
- Joshua D. Breidenbach (ORCID: https://orcid.org/0000-0002-5892-1879)
- Upasana Shrestha (ORCID: https://orcid.org/0000-0002-1249-4314)
- Prabhatchandra Dube (ORCID: https://orcid.org/0000-0003-2163-5788)
- Bella Khatib-Shahidi
- Sara Kazmi (ORCID: https://orcid.org/0000-0002-9379-6874)
- Dhilhani Faleel (ORCID: https://orcid.org/0000-0003-3234-8688)
- D.V. Malhotra (ORCID: https://orcid.org/0000-0002-0934-1560)
- Shereen Yassine (ORCID: https://orcid.org/0009-0007-9644-9652)
- Humza Bashir (ORCID: https://orcid.org/0009-0006-9921-6013)
- Caitlin Murphy (ORCID: https://orcid.org/0009-0007-2280-0518)
- Irum Syed (ORCID: https://orcid.org/0000-0002-0879-3450)
- Satkeerth Boyapalli
- Evan M. Benson
Institutions
- University of Toledo Medical Center (US)
- Los Alamos National Laboratory (US)
- University of Toledo (US)
Publication Details
- Journal
- Cells
- Published
- 2024-12-20
- DOI
- https://doi.org/10.3390/cells13242117
- Citations
- 4
- Primary Topic
- Genetic Syndromes and Imprinting
- Type
- article
- Field-Weighted Citation Impact
- 2.20
Funders
- National Science Foundation
- University of Toledo
- Ohio Department of Higher Education
- National Institutes of Health
- National Heart, Lung, and Blood Institute