The Sum of One’s Parts: Exploring Bone and Dental Age Assessment in Age Estimation Methods

Age estimation (AE) is a fundamental aspect used to establish the biological profile of both living and deceased individuals. This study evaluates AE methods to determine if bone development (BD) methods yield similar results to dental development (DD) and whether methods using samples with similar geographic origins, socioeconomic status (SES), chronology, data specificity, and/or anatomical regions yield consistent results. We hypothesized that BD and DD methods differ in age estimations, although these differences would be minor when methods have similar variables. The sample consisted of 11 immature skeletons from the Hospital Real de Todos os Santos’ collection (18th-century, Lisbon, Portugal) and applied 56 AE methods. The results were compiled into individual-based diagrams, facilitating both within- and between-individual comparisons, including stress-induced changes. This showed that BD methods tended to underestimate age compared to DD methods. BD methods closely aligning with DD methods were mainly based on individuals from lower to middle SES, focusing on areas like the iliac crest and medial clavicle. Findings also suggest that physiological stress might influence AE outcomes. This study emphasizes the importance of combining BD and DD methods alongside a detailed pathological and/or chronic stress assessment of human remains when estimating AE to minimize interpretative errors. This care applies to any discipline aiming to profile living or dead individuals, highlighting the importance of controlling for confounding variables, such as disease, in any AE estimation.