Immobilization of Candida tropicalis Lipase and Cells Isolated from Olive Pulp

Yeast isolated from waste olive pulp obtained from a local olive oil mill was identified and determined to be a Candida tropicalis strain. Lipase production optimization of C. tropicalis strain has been completed. The lipase produced according to the optimization parameters was partially purified by ammonium sulfate precipitation and dialysis. Crude enzyme, partially purified lipase, and cells of the C. torpicalis strain were separately immobilized into sodium alginate, k-Carrageenan, and Agar-Agar, respectively, and their lipase activities were investigated. The highest lipase activity was determined as 10.83 U/ml in the partially purified sample that was not immobilized.