In Vitro Biological Activity of Tiliacora triandra (Colebr.) Diels Root Extract

In this study, we explore the phytochemical properties of Tiliacora triandra (Colebr.) Diels, commonly known as Yanang, a herb traditionally used for treating fever and malaria. Our objective is to isolate and analyze bioactive compounds from T. triandra's root extract, with a specific focus on tiliacorinine (T1), assessing its antioxidant, and anticancer capabilities. The antioxidant activity of T. triandra extract (TTE) was evident, with IC50 values of 196.32 ± 10.51 µg/mL and 88.22 ± 1.95 µg/mL in the DPPH and ABTS assays, respectively. The results also demonstrated that TTE and T1 had a significant cytotoxic effect, with IC50 values varying in a concentration-dependent manner against A549 with the IC50 of 48.25 μg/mL and 26.38 μg/mL, respectively. Additionally, both TTE and T1 displayed anti-inflammatory effects, with the most effective inhibition of nitric oxide production observed at a concentration of 40 μg/mL. This study underlines the potential of T. triandra, and its compound tiliacorinine in developing treatments with antioxidant, anti-inflammatory, and anticancer properties.