Antishock effect of soybean extract during sperm cryopreservation of different animal species

The results of the study of the fortification effect on the plasma membranes of sperm from bulls, rams, and boars treated with soybean seed hydrolysate after washing and after exposure to temperature changes on sperm are presented. It was found that the lipoprotein extract from soybeans has the ability to protect germ cells from temperature shock under conditions of instantaneous temperature drop from 28°C to 0°C at the same level as native yolk. The direct dependence of the osmotic pressure in the extracts on the exposure temperature and extraction conditions was established. The replacement of native yolk in cryoprotective media with anti-shock components of plant origin ensures the preservation of high biological parameters of sperm after thawing. The use of a plant-derived plasma membrane fortifier instead of native yolk makes it possible to apply simple and reliable methods of sterilization, prevent contamination of sperm and female genital tract with yolk-transmitted pathogens, and thus increase the sanitary and hygienic level of artificial insemination