The Methodology of EPR Spectroscopy using in Analysis of Physical and Chemical Mechanisms of Radio-Genetics Damages in Animal and Human Organisms

The study of the complex of EPR characteristics of metabolic reactions involving free radicals and paramagnetic metal complexes revealed a pronounced dose-dependent dynamic SOS response of the most important life support systems of the body under General gamma irradiation. Studies in actively proliferating tissues of the ribonucleotide reductase enzyme using low-temperature EPR spectroscopy and parallel cytogenetic analysis of tissues and blood indicate initial radiation-induced trigger events that initiate the generation of DNA damage in hematopoietic organs. In the mechanism of the anti-radiation effect of radioprotectors, an increase in the activity of ribonucleotide reductase and the induction of deoxyribonucleotide synthesis is necessary for effective damage repair and DNA synthesis in cells of radiosensitive organs. The use of EPR biomarkers allowed us to justify the doses and modes of administration of radioprotectors to obtain optimal radio protection.