Enhancing D-lactic acid production by optimizing the expression of D-LDH gene in methylotrophic yeast Komagataella phaffii
This study demonstrated the effectiveness of combining different enzyme genes and promoters using multiple promoters with different induction and repression conditions, integrating the genes into the rDNA locus, and further amplifying the genes after transformation in K. phaffii. Using our established method, other K. phaffii strains can be engineered to produce various useful chemicals in the future.
Authors
- Ryosuke Yamada (ORCID: https://orcid.org/0000-0003-3820-7501)
- Takuya Matsumoto (ORCID: https://orcid.org/0000-0002-7258-5891)
- Hiroyasu Ogino (ORCID: https://orcid.org/0000-0002-5325-8289)
- Yoshifumi Inoue
Institutions
- Osaka Prefecture University (JP)
- Osaka Metropolitan University (JP)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2024-12-22
- DOI
- https://doi.org/10.1186/s13068-024-02596-0
- Citations
- 5
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 1.17
Funders
- Japan Society for the Promotion of Science