Green synthesis: silver nanoparticles from microbial extract for biogas generation

The primary objective of this study is the green synthesis of silver nanoparticles using the active extract obtained from microorganisms. The microorganism, a new strain of Bacillus hunanensis, was chosen from landfill leachate of Durgadi Fort dumping ground near Kalyan and soil near the decomposer machine of University of Mumbai, Vidyanagari campus. The new strain of Bacillus hunanensis (Accession Number: MK951982) culture was centrifuged and was sonicated for 10 min. The culture was once again centrifuged after being sonicated. The supernatant was decanted and distilled water was used to wash the precipitate. The process of producing silver nanoparticles involved reacting a standard silver salt solution with an aqueous bio-reducing solution obtained from the microorganism. The reaction progress was monitored by analytical techniques. The nanoparticles obtained were characterized by the FTIR, TEM and SEM-EDS. Further the nanoparticles produced were used in the digester for biogas emission.