Platelet rich plasma driven changes in hepatic glycogen metabolism and hepato-histological assessment in diabetic mice

Background: Platelet-rich plasma (PRP) emerges as a promising therapy, exhibiting noticeable anti-diabetic potential. Nevertheless, the precise underlying mechanism remains enigmatic. In the current investigation, we aimed to scrutinize the potential therapeutic ramifications of PRP in alloxan-induced diabetic mice, with a particular focus on its modulation of glycogen metabolism and glucose transport. Methods: Forty mice were randomly divided into four groups: NC (Control), PC (PRP treatment), DC (Alloxan dose), and DT (Alloxan+PRP treatment). PRP was administered subcutaneously over four weeks, with a frequency of twice-weekly dosing, to diabetic mice. Results: Our findings illustrate that PRP maintained glucose homeostasis through its modulatory influence on glycogen metabolism and glucose transporters (Glut2 and Glut4) on the hepatocyte membrane. PRP substantially reduced hepatic glycogen content significantly (P = 0.0148). Moreover, PRP treatment effectively preserved the structural integrity of hepatic lobules and maintained glycogen levels within the cells. There was a substantial increase in glycogen accumulation within the cytoplasm of liver cells in the diabetic group (DC). However, treatment with platelet-rich plasma (PRP) in the diabetes-treated (DT) group resulted in a marked improvement in glycogen accumulation within liver cells. Conclusion: In aggregate, we concluded that PRP has the potential to ameliorate hyperglycemia in DM by orchestrating the interplay of glycogen metabolism and glucose transport within the liver, ultimately restoring glycogen deposition in hepatic tissues.