A Modified Method for DNA Extraction From Buccal Cells for Genetic Epidemiological Studies Among Children
This protocol can be used to obtain DNA of high yield, purity, and functional quality from schoolchildren for genetic epidemiological studies based on PCR. This method is effective whether DNA is extracted immediately after collection or after buccal cell samples have been stored at 20°C for eight months.
Authors
- Nuria Patiño‐Marín (ORCID: https://orcid.org/0000-0003-0056-5901)
- Carlo Eduardo Medina‐Solís (ORCID: https://orcid.org/0000-0002-1410-9491)
- Nereyda Niño‐Martínez (ORCID: https://orcid.org/0000-0002-3240-7169)
- Wendy Yesenia Escobar de González (ORCID: https://orcid.org/0000-0001-9725-0783)
- Katleen Argentina Aguirre de Rodríguez (ORCID: https://orcid.org/0000-0002-9200-4214)
- Terán-Figueroa Yolanda
- Vianney Castañeda Monroy
Institutions
- Universidad Autónoma del Estado de Hidalgo (MX)
- Polytechnic University of San Luis Potosí (MX)
- Autonomous University of Yucatán (MX)
- University of El Salvador (SV)
Publication Details
- Journal
- Cureus
- Published
- 2024-12-21
- DOI
- https://doi.org/10.7759/cureus.76140
- Primary Topic
- Digestive system and related health
- Type
- article
- Field-Weighted Citation Impact
- 0.00