Investigation of Potential Food Intake Biomarkers by LC‐MS/MS: Suitability Under Conditions Close to Everyday Live

The use of different biomarkers enables the evaluation of different eating habits without any prior wash out period or bigger than usual portion sizes. Especially long-term biomarkers seem to have a good suitability. Very high intakes and very low intakes are easier to distinguish than a moderate intake. Food intake biomarkers are proposed as a more reliable tool than food diaries or food frequency questionnaires. Nevertheless, data under realistic conditions are rarely available. In this study, ten volunteers were divided into three groups with different meal plans. An LC-MS/MS approach was performed and the data were further investigated with the main goal of grouping the participants according their provided diet, while the first author did not have any knowledge about the meal plans.