Comparative Efficiency of Different Culture Media on in vitro Embryonic Development of Goat Oocytes

Background: The study was conducted to compare the development of goat embryos in simple media with BSA and complex culture media supplemented with serum on the development of goat embryos. Also, studied the effect of oviductal cell co-culture on the developmental competence of goat oocytes. Methods: The A and B quality goat oocytes subjected to in vitro maturation in TCM 199 + 10% FCS + FSH + Estradiol for 24 hours in a CO2 incubator (6% CO2) at 38.5°C. The oocytes that exhibited cumulus expansion subjected to in vitro fertilization in 1) BO - IVF and 2) TALP-fertilization media supplemented with heparin. The cleavage percent found significantly higher in BO-IVF (66.66%) compared to TALP- fertilization media (42.07%). After 18 hours of sperm-oocyte incubation, the presumptive zygotes from BO-IVF media divided randomly into 3 groups and cultured in one of the following 3 media, group 1) CR1aa media; group 2) BO-IVC media and group 3) TCM-199 + 10% FBS. Result: The cleavage percentage was significantly higher (P greater than 0.05) in BO-IVC media than CR1aa media and TCM-199 media (72.32, 64.35 and 35.23 percent respectively). The blastocyst development observed significantly higher (P greater than 0.05) in CR1aa compared to commercial BO-IVC media and TCM-199 media (9.23, 5.55, and 4.05 percent, respectively). Among the different media compared, CR1aa and BO-IVC media were effective for supporting the development of goat zygotes to the morula and blastocyst stages than those obtained when a complex TCM-199 medium used with serum supplementation. The effect of oviductal cell co-culture on the developmental competence of goat oocytes studied. The blastocyst development rate found higher in CR1aa + oviductal and TCM- 199 + oviductal cells as compared to CR1aa and TCM-199 alone.