High Fat Diet Mediated Inflammatory molecules Induced development OF Diabetogensis In Rats Ameliorated by Hesperidin.

High fat diet rich in saturated fatty acids caused serious health problems. Citrus fruits rich in flavonoids as Hesperidin that exerts different biological activities as anticancer. The wastes produced during food processing yield a huge amount of citrus residue, these wastes are a good source of hesperidin. This study investigated the complications of high fat diet (HFD) in development of metabolic syndrome as insulin resistance and evaluated the role of hesperidin to protect against these abnormalities. To achieve this purpose , four groups of rats were included, Gr I: Control; Gr II: rats treated with 30 mg hesperidin /kg bw/day; Gr III: HFD rats fed on diet contain 45 % lard fat and Gr IV:HFD treated with 30 mg hesperidin /kg bw/day. After 3 months, fasting sugar, HA1c, insulin, HOMA-IR, lipid profile, malondialdehyde, antioxidant activities, TNF-α and IL-1β were evaluated. Data obtained showed that, rats fed HFD diet showed a significant elevation of the levels of HA1c, MDA, LDL-c, TG, TNF-α and IL-1β while antioxidant enzymes activities were significantly decreased in rats fed HF versus control (p<0.001). Hesperidin improve HOMA-IR, decreased LDL-c, TG, increased antioxidant activity and decreased MDA. The levels of TNF-α and IL-1β were significantly lowered in rats treated with hesperidin versus untreated group (p<0.001). In conclusion, hesperidin ameliorated abnormalities induced by HFD diet as indicated by insulin sensitivity, enhanced antioxidant activity and reduced inflammatory mediators release.