Molecular Analysis of Escherichia coli and Correlations Between Phylogroups and Sequence Types from Different Sources

<i>Escherichia coli</i> is a significant pathogen responsible for infections in both humans and livestock, possessing various virulence mechanisms and antimicrobial resistance that make it even more concerning. In this study, several internationally recognized clones of <i>E. coli</i> were identified, such as ST131, ST38, ST648, and ST354, from chicken meat, pork, and human infection samples. Notably, ST131, belonging to phylogroup B2, was the dominant sequence type (ST) in human samples, while ST38, belonging to phylogroup D, was the most prevalent in meat samples. Several antibiotic resistance genes were identified: the <i>gyrA</i> gene mutation was the most prevalent, and CTX-M-55 was the most common extended-spectrum beta-lactamases (ESBLs), with significant differences noted for CTX-M-2 and CTX-M-15. Virulence-associated genes (VAGs) such as <i>gad</i> and <i>iss</i> were frequently found, especially in human isolates. These findings highlight the complex epidemiology of antibiotic-resistant <i>E. coli</i> in community settings and the potential risks associated with commercial meat.