Multiplex allele-specific PCR and DNA chromatography to identify genotypes of Salmonella enterica serovar Typhimurium and its monophasic variants
We constructed a simple genotyping method combining multiplex allele-specific PCR and DNA chromatography for Salmonella enterica subsp. enterica serovar Typhimurium and its monophasic variant. The developed method can be used to estimate the genetic background of isolates, and it facilitates easy identification of several epidemic clades among these serotypes.
Authors
- Nobuo Arai (ORCID: https://orcid.org/0000-0003-2285-1597)
- Ayako Watanabe-Yanai
- Masahiro Kusumoto (ORCID: https://orcid.org/0000-0001-8675-4732)
- Yukino Tamamura-Andoh (ORCID: https://orcid.org/0000-0002-1839-0655)
- Taketoshi Iwata
Institutions
- National Agriculture and Food Research Organization (JP)
- National Institute of Animal Health (JP)
Publication Details
- Journal
- Journal of Microbiological Methods
- Published
- 2024-12-20
- DOI
- https://doi.org/10.1016/j.mimet.2024.107082
- Primary Topic
- Salmonella and Campylobacter epidemiology
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Japan Society for the Promotion of Science