Risk assessment of oligomers and potentially harmful chemical compounds from food packaging simulated using an in vitro digestion model

The objective of this study, PLASTOGEST, was to evaluate the risk associated with oligomers and other potentially harmful chemical compounds when consumed by humans through food. This research systematically reviewed existing literature and applies untargeted analysis to assess the fate of non-intentionally added substances (NIAS) and intentionally added substances (IAS) during in vitro digestion. Polyethylene terephthalate and polybutylene terephthalate oligomers and simulation of real food conditions were used to evaluate migration and how these oligomers behave during in vitro digestion. This approach, involving enzymes and digestive juices, measured bioavailable levels of these substances in humans. The analytical work, including sample preparation was performed at Aarhus University, whilst extraction and untargeted analysis using ultra-high performance liquid chromatography trap ion mobility spectroscopy time-of-flight mass spectrometry (UHPLC-TIMS/TOF-MS), were performed at Aristotle University of Thessaloniki. The EUFORA fellow gained insights into advanced analytical techniques and risk assessment, following specific EFSA guidelines. The study aimed to enhance our understanding on how plastic packaging materials might affect human health, potentially leading to more rigorous risk assessment frameworks.