Determination of the effective concentration of phytohormones at the stages of initiation and micropropagation of some rootstocks

The article presents the results of laboratory studies conducted to evaluate the effectiveness of nutrient media with different hormonal compositions in the process of initiation and micropropagation in the in vitro culture of clonal rootstocks MaxMa 14, Myrobalan 29C, GF 677, Garnem 15 and OHF 87. In the study, the effectiveness of nutrient media with different concentrations and combinations of phytohormones was studied step by step. It was found that the modified media made it possible to obtain a relatively high result at the stage of micropropagation. Thus, the maximum reproduction coefficient of microcuttings of rootstocks according to the variants of nutrient media was 5.82-6.27. The modified composition of nutrient media significantly increased the multiplication coefficient of microcuttings of rootstocks of fruit crops at the stage of micropropagation, which, of course, will have a positive result on the outcome of the entire process of micropropagation of these rootstocks in the in vitro culture.