Conformational changes in and translocation of small proteins: insights into the ejection mechanism of podophages
Podophage T7 core proteins form an elongated trans-envelope channel for genomic DNA delivery into the host cell. The structures of the core proteins within the mature T7 and assembled in the periplasmic tunnel form in the DNA-ejected T7 have been resolved previously. Here, we resolved the structures of two new structural proteins (gp6.7 and gp7.3) within mature T7 and receptor-induced DNA-ejected T7. The gp6.7 protein participates in the assembly of the core complex within mature T7 and the trans-envelope channel during T7 infection; therefore, gp6.7 is a core protein. Before T7 infection, gp7.3 plays a role in promoting the assembly of the nozzle into the adaptor.
Authors
- Hao Xiao (ORCID: https://orcid.org/0000-0003-2461-494X)
- Jingdong Song (ORCID: https://orcid.org/0000-0001-8808-1981)
- Hongrong Liu (ORCID: https://orcid.org/0000-0001-6247-5464)
- Lingpeng Cheng (ORCID: https://orcid.org/0000-0002-9148-0550)
- Wenyuan Chen (ORCID: https://orcid.org/0000-0002-9449-8596)
- Li Wang (ORCID: https://orcid.org/0000-0002-2929-4255)
- Jing Zheng (ORCID: https://orcid.org/0000-0003-1747-5398)
- Hao Pang (ORCID: https://orcid.org/0000-0002-6606-6438)
Institutions
- Hunan Normal University (CN)
- Taiwan Centers for Disease Control (TW)
- National Institute for Viral Disease Control and Prevention (CN)
Publication Details
- Journal
- Journal of Virology
- Published
- 2024-12-20
- DOI
- https://doi.org/10.1128/jvi.01249-24
- Citations
- 11
- Primary Topic
- Galectins and Cancer Biology
- Type
- article
- Field-Weighted Citation Impact
- 2.65
Funders
- National Natural Science Foundation of China
- National Science and Technology Major Project