BIL7 enhances plant growth by regulating the transcription factor BIL1/BZR1 during brassinosteroid signaling<sup>‡</sup>
Brassinosteroids (BRs) are plant steroid hormones that regulate plant development and environmental responses. BIL1/BZR1, a master transcription factor that regulates approximately 3000 genes in the BR signaling pathway, is transported to the nucleus from the cytosol in response to BR signaling; however, the molecular mechanism underlying this process is unknown. Here, we identify a novel BR signaling factor, BIL7, that enhances plant growth and positively regulates the nuclear accumulation of BIL1/BZR1 in Arabidopsis thaliana. BIL7-overexpressing plants were resistant to the BR biosynthesis inhibitor Brz and taller than wild-type (WT) plants were due to increased cell division. BIL7 is mainly localized to the plasma membrane, but during the early stages of cell growth, it was also localized to the nucleus. BIL7 was directly phosphorylated by the kinase BIN2, and nuclear localization of BIL7 was enhanced by the BIN2 inhibitor bikinin. BIL7 was found to bind to BIL1/BZR1, and nuclear accumulation of BIL1/BZR1 was strongly enhanced by BIL7 overexpression. Finally, double overexpression of BIL1/BZR1 and BIL7 led to greatly elongated hypocotyls in the presence of Brz. These findings suggest that BIL7 mediates nuclear accumulation of BIL1/BZR1, which activates inflorescence elongation in plants via BR signaling.
Authors
- Ayumi Yamagami (ORCID: https://orcid.org/0000-0003-4222-4769)
- Shohei Nosaki (ORCID: https://orcid.org/0000-0001-8493-1299)
- Masaru Tanokura (ORCID: https://orcid.org/0000-0001-5072-2480)
- Mariano García-Hourquet (ORCID: https://orcid.org/0000-0002-1313-2139)
- Takuya Miyakawa (ORCID: https://orcid.org/0000-0001-5957-3549)
- Tomoko Miyaji
- Ryo Tachibana (ORCID: https://orcid.org/0000-0002-8265-7502)
- Surina Surina (ORCID: https://orcid.org/0009-0000-1948-9748)
- Hiroyuki Osada (ORCID: https://orcid.org/0000-0002-3606-4925)
- Tadao Asami (ORCID: https://orcid.org/0000-0003-0774-6203)
- Minami Matsui (ORCID: https://orcid.org/0000-0001-5162-2668)
- Takeshi Nakano (ORCID: https://orcid.org/0000-0002-3188-7770)
- Santiago Mora‐García (ORCID: https://orcid.org/0000-0002-4741-1515)
- Yusuke Nakamura (ORCID: https://orcid.org/0000-0002-2310-0587)
- Kaisei Nishida (ORCID: https://orcid.org/0009-0003-1426-4736)
- Kazuo Shinozaki (ORCID: https://orcid.org/0000-0002-6317-9867)
- Shozo Fujioka
Institutions
- Kihara Institute for Biological Research (JP)
- University of Tsukuba (JP)
- Kyoto University (JP)
- Fundación Instituto Leloir (AR)
- RIKEN Center for Sustainable Resource Science (JP)
- Microbial Chemistry Research Foundation (JP)
- Synthetic Genomics (United States) (US)
- The University of Tokyo (JP)
- Yokohama City University (JP)
Publication Details
- Journal
- The Plant Journal
- Published
- 2024-12-20
- DOI
- https://doi.org/10.1111/tpj.17212
- Citations
- 4
- Primary Topic
- Plant Molecular Biology Research
- Type
- article
- Field-Weighted Citation Impact
- 2.40
Funders
- Japan Society for the Promotion of Science
- Bio-oriented Technology Research Advancement Institution
- Core Research for Evolutional Science and Technology