Multiscale multi-functional phase imaging platform for cell and tissue characterization

Phase Imaging offers a label-free approach to non-invasively characterize cellular processes and tissue architecture by exploiting their refractive index based intrinsic contrast in their natural states. In this study, we present a multiscale phase imaging system that bridges mesoscale and nanoscale imaging. This system shows the capability for large-scale, simultaneous tracking of live-cell morphodynamics, capturing sub-cellular details across thousands of cells as they develop resistance to chemotherapy drugs and the ability to reveal sub-cellular features in pathological tissue.