Skin-iDISCO+: An optimized tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature
Tissue clearing enables deep imaging of long biological structures using light microscopy approaches. Protocols such as iDISCO are not currently available for human skin. Here, we present Skin-iDISCO<sup>+</sup>, a tissue-clearing and labeling protocol for morphometric analysis of human cutaneous vasculature. We describe steps for processing and pre-treating tissue, immunostaining, clearing, and imaging using confocal and light-sheet fluorescence microscopy. We then detail procedures for the reconstruction of networks and the extraction of morphometric parameters of the skin vasculature. For complete details on the use and execution of this protocol, please refer to Renier et al.<sup>1</sup>.
Authors
- Muriel Cario (ORCID: https://orcid.org/0000-0003-0462-5684)
- Jérémie Teillon (ORCID: https://orcid.org/0000-0002-5335-6153)
- Hamid Rezvani (ORCID: https://orcid.org/0000-0001-8067-1338)
- François Moisan (ORCID: https://orcid.org/0000-0003-2680-7497)
- Sébastien Marais (ORCID: https://orcid.org/0000-0003-1521-1188)
- L. Pechtimaldjian
- Sébastien Dupichaud
Institutions
- Université de Bordeaux (FR)
- Inserm (FR)
- Bordeaux Population Health (FR)
- Carl Zeiss (United States) (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2024-12-20
- DOI
- https://doi.org/10.1016/j.xpro.2024.103524
- Citations
- 2
- Primary Topic
- Cellular Mechanics and Interactions
- Type
- article
- Field-Weighted Citation Impact
- 0.65