Growth-Inhibitory Effects of Traditional Chemotherapeutic Combinations (FOLFOX, FOLFIRI) on Colon Cancer Cells: An in vitro Study

Objective: To compare the in vitro growth-inhibitory effects of the commonly used chemotherapeutic combinations folinic acid, 5-fluorouracil, and oxaliplatin (FOLFOX) and folinic acid, 5-fluorouracil, and irinotecan (FOLFIRI) on colon cancer cells (HT29 and CaCo-2 cells). Materials and Methods: The viability of HT29 and CaCo-2 cells treated with different concentrations of the FOLFOX and FOLFIRI combinations was evaluated using the MTT (3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide) assay. Results: FOLFOX and FOLFIRI combinations exhibited varying effects on the colon cancer cell lines, with HT29 cells showing sensitivity, while CaCo-2 cells demonstrated resistance to these treatments. Conclusions: The results of this preliminary study will contribute to the development of effective and targeted clinical treatment strategies for colon cancer.