Time-of-flight widefield microscopy

High-resolution depth imaging is essential in fields such as biological microscopy and material science. Traditional techniques like interferometry and holography often rely on phase stability and coherence, making them susceptible to noise and limiting their effectiveness in low-light conditions. We propose a time-of-flight (ToF) widefield microscopy technique that uses pseudo-thermal light. Our method calculates second-order correlation <i>g</i> <sup>(2)</sup> from intensity images captured by a standard CMOS camera, eliminating the need for phase stability and improving robustness in noisy environments. By incorporating a dual-wavelength approach, we further enhance depth-uncertainty to nanometer scales. We demonstrate the effectiveness of this technique through experiments on biological samples and nano-scale graphene layers, highlighting its potential for high-precision depth-resolved imaging.

Authors

Publication Details

Journal
Optics Express
Published
2024-12-20
DOI
https://doi.org/10.1364/oe.542259
Citations
2
Primary Topic
Advanced Optical Sensing Technologies
Type
article
Field-Weighted Citation Impact
1.01

Funders

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article

Time-of-flight widefield microscopy

Khaled Kassem, Philip Binner, Ashley Lyons, Daniele Faccio
2 citations
Optics Express
Advanced Optical Sensing Technologies
1.01
article

Time-of-flight widefield microscopy

Khaled Kassem, Philip Binner, Ashley Lyons, Daniele Faccio
article en
2 citations

Abstract

High-resolution depth imaging is essential in fields such as biological microscopy and material science. Traditional techniques like interferometry and holography often rely on phase stability and coherence, making them susceptible to noise and limiting their effectiveness in low-light conditions. We propose a time-of-flight (ToF) widefield microscopy technique that uses pseudo-thermal light. Our method calculates second-order correlation <i>g</i> <sup>(2)</sup> from intensity images captured by a standard CMOS camera, eliminating the need for phase stability and improving robustness in noisy environments. By incorporating a dual-wavelength approach, we further enhance depth-uncertainty to nanometer scales. We demonstrate the effectiveness of this technique through experiments on biological samples and nano-scale graphene layers, highlighting its potential for high-precision depth-resolved imaging.

Optics ExpressVol. 33(1)
UK Research and Innovation, Royal Academy of Engineering, Engineering and Physical Sciences Research Council
Openalex Percentile: Top 18%
Advanced Optical Sensing Technologies
1.01
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