Transcriptomic analysis of male diamondback moth antennae: Response to female semiochemicals and allyl isothiocyanate

Female semiochemicals and allyl isothiocyanate (AITC) attract moths, and the moths use odorant-degrading enzymes (ODEs) to break down the excess odor. By identifying antennae-specific ODEs, researchers have established the molecular foundation for odorant degradation and signal inactivation in insects. This enables further exploration of new pest control methods. Currently, the degradation of female semiochemicals and AITC has received limited attention, inspiring this study to identify target ODEs in diamondback moths through transcriptome analysis. Sequencing of antennae from male adults (MA) exposed to female adults (FA) and AITC yielded a substantial 54.18 Gb of clean data, revealing 2276 differentially expressed genes (DEGs) between the MA and MA-FA treatments, and 629 DEGs between MA and MA-AITC treatments. The analysis of MAs exposed to FAs and AITC identified 29 and 17 ODEs, respectively, mainly involving aldehyde dehydrogenases (ALDHs), alcohol dehydrogenases (ADs), cytochrome P450s (CYPs), and UDP-glucuronosyltransferases (UGTs). Pathway analysis revealed primary enrichment in glycolysis/gluconeogenesis and fatty acid degradation in female adult treatments. In contrast, AITC treatments showed major enrichment in pathways related to pentose and glucuronate interconversions, retinol metabolism, and ascorbate and aldarate metabolism. Additionally, qRT-PCR analysis validated the expression patterns of 10 ODE genes in response to these treatments, with varying results observed among the genes. These findings indicate significant changes in ODE expression levels, providing a molecular foundation for identifying potential targets for behavioral inhibitors.