Exploiting functional regions in the viral RNA genome as druggable entities
RNA-targeting compounds or small interfering RNAs (siRNAs) offer a potent means to control viral infections. An essential prerequisite for their design depends on identifying conserved and functional viral RNA structures in cells. Techniques that probe RNA structures in situ have recently been developed including SHAPE-MaP, which has been helpful in the analysis of secondary structures of RNA. In this study, we report on the application of SHAPE-MaP to the porcine epidemic diarrhea virus RNA genome to categorize different functional regions, including potential quadruplex-forming sequence and target sites of siRNA. Our results show that these structures can be exploited to inhibit viral proliferation and that SHAPE-MaP is an effective method to identify secondary structures in RNA genomes.
Authors
- Yingge Zheng
- Dengguo Wei (ORCID: https://orcid.org/0000-0001-9923-789X)
- Shozeb M Haider (ORCID: https://orcid.org/0000-0003-2650-2925)
- Dehua Luo
- Zi Wen (ORCID: https://orcid.org/0000-0001-7600-4399)
- Lijun Guo (ORCID: https://orcid.org/0009-0005-5189-415X)
- Zhiyuan Huang (ORCID: https://orcid.org/0000-0003-1284-2128)
- Qingling Li (ORCID: https://orcid.org/0000-0002-5266-5210)
- Yuqing Bai (ORCID: https://orcid.org/0009-0004-1428-3459)
- Yingxiang Deng
Institutions
- Huazhong Agricultural University (CN)
- University College London (GB)
- University of Tabuk (SA)
Publication Details
- Journal
- eLife
- Published
- 2024-12-19
- DOI
- https://doi.org/10.7554/elife.103923
- Citations
- 3
- Primary Topic
- RNA and protein synthesis mechanisms
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- National Natural Science Foundation of China
- China Postdoctoral Science Foundation
- Natural Science Foundation of Hubei Province
- Huazhong Agricultural University
- Fundamental Research Funds for the Central Universities