Formation of Complex Spatial Structure of DNA in the Process of ab initio Synthesis

Products with a complex spatial organization are formed during the ab initio synthesis under the action of Bst DNA polymerase, large fragment. Using the analysis of AFM images of the products obtained with the addition of a minimal amount of the nicking endonuclease Nt. BspD6I during this synthesis, it became possible to describe the structures formed as a result of this synthesis and suggest that their formation occurs by the mechanism of replication-dependent recombination.