MVA-based SARS-CoV-2 vaccine candidates encoding different spike protein conformations induce distinct early transcriptional responses which may impact subsequent adaptive immunity
Our findings suggest a potential impact of the spike protein conformation not only on adaptive but also on innate immune responses. As indicated by positive correlations between several immune parameters induced by MVA-SARS-2-ST, the distinct transcriptional activation early after vaccination may be linked to the induction of classical monocytes and activation of cTFH1 cells, which may in turn result in the superior adaptive immunogenicity of MVA-SARS-2-ST, compared to MVA-SARS-2-S. Overall, our data demonstrate that both the vaccine platform and antigen insert can affect innate immune responses and subsequent vaccine immunogenicity in humans.
Authors
- M. Claudia tom Dieck (ORCID: https://orcid.org/0000-0002-8765-8969)
- Michael Spohn
- Leonie Marie Weskamm (ORCID: https://orcid.org/0000-0002-1175-7354)
- Anahita Fathi (ORCID: https://orcid.org/0000-0002-9351-9471)
- Leonie Mayer (ORCID: https://orcid.org/0000-0001-7102-4358)
- Marylyn M. Addo (ORCID: https://orcid.org/0000-0003-2836-9224)
- Asisa Volz (ORCID: https://orcid.org/0000-0001-8757-3896)
- Ilka Grewe (ORCID: https://orcid.org/0000-0002-9714-0725)
- My Linh Ly
- Christine Dahlke (ORCID: https://orcid.org/0000-0002-2056-9195)
- Monika Rottstegge
- Monika Friedrich
- Sibylle C. Mellinghoff (ORCID: https://orcid.org/0000-0003-3928-2503)
- Stephan Becker
- Verena Krähling
- Rebekka Kraemer
Institutions
- Universität Hamburg (DE)
- Philipps University of Marburg (DE)
- University of Cologne (DE)
- University of Veterinary Medicine Hannover, Foundation (DE)
- Christian-Albrechts-Universität zu Kiel (DE)
- Düsseldorf University Hospital (DE)
- University Medical Center Hamburg-Eppendorf (DE)
- Kinderkrebs-Zentrum Hamburg (DE)
- German Center for Infection Research (DE)
- Cologne Excellence Cluster on Cellular Stress Responses in Aging Associated Diseases (DE)
- University Hospital Cologne (DE)
- Heinrich Heine University Düsseldorf (DE)
- Bernhard Nocht Institute for Tropical Medicine (DE)
Publication Details
- Journal
- Frontiers in Immunology
- Published
- 2024-12-19
- DOI
- https://doi.org/10.3389/fimmu.2024.1500615
- Citations
- 4
- Primary Topic
- SARS-CoV-2 and COVID-19 Research
- Type
- article
- Field-Weighted Citation Impact
- 1.56
Funders
- Clinical Trial Center, China Medical University Hospital
- Joachim Herz Stiftung
- Deutsches Zentrum für Infektionsforschung
- Deutsche Forschungsgemeinschaft
- Universitätsklinikum Hamburg-Eppendorf