Extracellular vesicles from platelet-poor plasma possess anti-inflammatory and anti-catabolic effects in chondrocytes stimulated with IL-1β or synovial membrane-conditioned media
In this study, we confirmed that PPP is a suitable source of EVs, which can be efficiently isolated by SEC. We found that PPP-EVs were capable of decreasing the expression of inflammatory genes in OA chondrocytes stimulated with IL-1β or CM/SM. This study provides preliminary results on PPP-EVs as an affordable and promising option to modulate the catabolic microenvironment of OA chondrocytes in vitro.
Authors
- Denisa Harvanová (ORCID: https://orcid.org/0000-0002-3038-7520)
- Slavomira Gulová (ORCID: https://orcid.org/0000-0001-8911-3199)
- Marko Morávek (ORCID: https://orcid.org/0000-0002-9795-0170)
- Marek Lacko (ORCID: https://orcid.org/0000-0002-4276-3722)
- Jana Janočková (ORCID: https://orcid.org/0000-0002-2034-1860)
- Jana Matejová (ORCID: https://orcid.org/0000-0002-9086-3621)
- Lívia Kolesár Fecskeová (ORCID: https://orcid.org/0000-0002-0752-2173)
- Lucia Slovinská (ORCID: https://orcid.org/0000-0002-9945-0655)
Institutions
- University of Pavol Jozef Šafárik (SK)
Publication Details
- Journal
- Journal of Orthopaedic Surgery and Research
- Published
- 2024-12-19
- DOI
- https://doi.org/10.1186/s13018-024-05355-x
- Citations
- 3
- Primary Topic
- Extracellular vesicles in disease
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- Agentúra na Podporu Výskumu a Vývoja
- Vedecká Grantová Agentúra MŠVVaŠ SR a SAV