Molecular Imaging of Ovarian Follicles and Tumors With Near‐Infrared II Bioconjugates
Follicular tracking is typically conducted using ultrasound technology, but its effectiveness is constrained by limited resolution. High-resolution imaging of deep tissues can be accomplished using luminescence imaging in the near-infrared II window (NIR-II, 1000-1700 nm); however, the contrast agents that are used lack specificity. Here, it is reported that the FDA-approved indocyanine green (ICG)-conjugated recombinant human chorionic gonadotropin (hCG) protein can target early follicles with long-term effectiveness. A novel high-resolution NIR-II imaging approach is developed for monitoring follicular development as well as ovulation using multi-color imaging of ovarian vessels with a combination of non-overlapping downconversion nanoparticles (DCNPs). The results showed that the ability to monitor early follicles of around 50 µm in diameter exceeded the spatial and temporal resolution of ultrasound or MRI without the reproductive damage associated with computed tomography radiation, and this enabled the clinical identification of the follicular reserve in patients with infertility diseases such as polycystic ovary syndrome (PCOS). In addition, NIR-II imaging clearly targeted ovarian tumors and showed micro-metastatic lesions, thus providing a new tool for monitoring tumors in vivo and guiding surgical resection.
Authors
- Wenhao Gao (ORCID: https://orcid.org/0009-0007-7928-2678)
- Yi Feng (ORCID: https://orcid.org/0000-0002-7156-6023)
- Zi‐Han Chen (ORCID: https://orcid.org/0000-0002-3322-1489)
- Mingzhen Xu (ORCID: https://orcid.org/0000-0003-4011-8495)
- Kuinyu Lee (ORCID: https://orcid.org/0000-0002-2701-4037)
- Wenhan Lu (ORCID: https://orcid.org/0000-0003-3890-5553)
- Xiaoyu Tong (ORCID: https://orcid.org/0000-0002-1343-6335)
- Hao Chen (ORCID: https://orcid.org/0000-0002-8470-5467)
- Zhiming Wang (ORCID: https://orcid.org/0000-0002-0201-8912)
- Fan Zhang (ORCID: https://orcid.org/0000-0001-7886-6144)
- Yan Xiao (ORCID: https://orcid.org/0009-0009-2281-4375)
- Wei Hu (ORCID: https://orcid.org/0000-0001-8813-633X)
- Ruihu Song (ORCID: https://orcid.org/0000-0002-1606-9198)
- Zhao Fang (ORCID: https://orcid.org/0000-0003-3057-8738)
- Zhenle Pei (ORCID: https://orcid.org/0000-0002-8921-2872)
- Yu Wang (ORCID: https://orcid.org/0000-0003-1077-9305)
- Yicong Wang (ORCID: https://orcid.org/0000-0001-9102-4363)
Institutions
- Shanghai Medical College of Fudan University (CN)
- Chinese Academy of Sciences (CN)
- Fudan University (CN)
- Fudan University Shanghai Cancer Center (CN)
- Eye & ENT Hospital of Fudan University (CN)
- Shanghai Institute of Materia Medica (CN)
- Obstetrics and Gynecology Hospital of Fudan University (CN)
- China Association of Acupuncture-Moxibustion (CN)
- State Key Laboratory of Medical Neurobiology
Publication Details
- Journal
- Advanced Materials
- Published
- 2024-12-18
- DOI
- https://doi.org/10.1002/adma.202414129
- Citations
- 8
- Primary Topic
- Ultrasound and Hyperthermia Applications
- Type
- article
- Field-Weighted Citation Impact
- 1.48
Funders
- National Natural Science Foundation of China