The potential effect of irisin in endotoxemia induced cardiac injury in mouse model: role of AMPK

Polymicrobial sepsis is a life-threatening situation characterized by multiorgan dysfunction resulting from the body's abnormal response to microbial invasion. The assessment of irisin cardio protective potential against experimentally sepsis-caused endotoxic cardiac damage in mice was the aim of research. 24 Mice were enrolled in separated four groups (n = 6): Sham group, CLP group, ddH2O group, Irisin-treated group 10μg/kg IP, 1hr before CLP. 24 hr later, all mice were sacrificed and a cardiac sample was taken for measurement of TNF-α, MPO, caspase-11, F2 – isoprostane and serum troponin by ELISA and gene expression of AMPK by qpcr and histopathological study. Results: irisin treated group showed significant changes as compared with CLP group regarding TNF-α, MPO, CASPASE-11, F2 – ISOPROSTANE and (Ctn-I) as well as affect tissue mRNA expression of AMPK gene (p<0.05). We conclude that Irisin has cardio protective effects attributable to its anti-inflammatory and anti-oxidative action. Also, Irisin showed a cardio-protective effect as they affect tissue mRNA expression of AMPK gene.