An Improved P(V) Thio-Oligonucleotide Synthesis Platform
Three critical advances in simplifying the adoption of P(V)-based stereopure, phosphorothioate-containing oligonucleotide synthesis are reported. A more inexpensive phosphorus-sulfur incorporation reagent (<b>Ψ</b><sup><b>Br</b></sup>) is introduced, a robust linker system was developed, and a systematic study of common nucleobase protecting groups was performed to significantly reduce the barrier to adoption of this technology.
Authors
- Luca Gherardi (ORCID: https://orcid.org/0009-0005-0825-8377)
- Kyle W. Knouse (ORCID: https://orcid.org/0000-0001-9688-0513)
- Yao Feng (ORCID: https://orcid.org/0000-0001-7923-8158)
- Richard L. Redman (ORCID: https://orcid.org/0000-0002-7091-0988)
- Phil S. Baran (ORCID: https://orcid.org/0000-0001-9193-9053)
- Arjun Natarajan
- Molhm Nassir (ORCID: https://orcid.org/0000-0002-4996-2249)
- Yang Yang (ORCID: https://orcid.org/0000-0002-8190-1825)
- Yonghao Jin (ORCID: https://orcid.org/0000-0003-2346-8768)
- Nicholas Raheja
- David Butler
Institutions
- University of Pisa (IT)
- Scripps Institution of Oceanography (US)
- Torrey Pines Institute For Molecular Studies (US)
- Scripps (United States) (US)
- Viva Biotech (China) (CN)
Publication Details
- Journal
- Organic Letters
- Published
- 2024-12-19
- DOI
- https://doi.org/10.1021/acs.orglett.4c03980
- Citations
- 3
- Primary Topic
- Advanced biosensing and bioanalysis techniques
- Type
- article
- Field-Weighted Citation Impact
- 0.70
Funders
- Council for Higher Education
- National Institutes of Health
- National Institute of General Medical Sciences