Design of primer and linear probe sets for swine and wild boar HEV detection by PCR

Hepatitis E virus (HEV) infects humans and several mammals and it has 8 genotypes (HEV1 HEV8). HEV1 4 is the causative agent of Hepatitis E in humans. HEV5 6 was detected only in wild boar in Japan, HEV7 8 was detected in camels. HEV3 4 is characterized by zoonotic potential. Swines and wild boars are main natural reservoirs for this virus. Besides, HEV 3 was detected in deers, dolphins, rabbits, cattle, goats that is additional risk for virus interspecies transmission from domestic animals to humans. Availability of mismatched nucleotides in the complexes of primer/probe with viral targets was applied for estimation of primer sets. Based on determined conserved fragments two sets with LNA containing primer and linear LNA containing probe without mismatched nucleotides in the complexes of primer/probe with single stranded amplicon for real time reverse transcriptase PCR detection of swine and wild boar HEV 3 and HEV 4 isolates were designed. Primer sets may be used for HEV detection by standard PCR

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Publication Details

Journal
Veterinary Medicine inter-departmental subject scientific collection
Published
2024-10-23
DOI
https://doi.org/10.36016/vm-2024-110-9
Primary Topic
Hepatitis Viruses Studies and Epidemiology
Type
article
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article

Design of primer and linear probe sets for swine and wild boar HEV detection by PCR

O. Yu. Limanskaya
Veterinary Medicine inter-departmental subject scientific collection
Hepatitis Viruses Studies and Epidemiology
article

Design of primer and linear probe sets for swine and wild boar HEV detection by PCR

O. Yu. Limanskaya
article en

Abstract

Hepatitis E virus (HEV) infects humans and several mammals and it has 8 genotypes (HEV1 HEV8). HEV1 4 is the causative agent of Hepatitis E in humans. HEV5 6 was detected only in wild boar in Japan, HEV7 8 was detected in camels. HEV3 4 is characterized by zoonotic potential. Swines and wild boars are main natural reservoirs for this virus. Besides, HEV 3 was detected in deers, dolphins, rabbits, cattle, goats that is additional risk for virus interspecies transmission from domestic animals to humans. Availability of mismatched nucleotides in the complexes of primer/probe with viral targets was applied for estimation of primer sets. Based on determined conserved fragments two sets with LNA containing primer and linear LNA containing probe without mismatched nucleotides in the complexes of primer/probe with single stranded amplicon for real time reverse transcriptase PCR detection of swine and wild boar HEV 3 and HEV 4 isolates were designed. Primer sets may be used for HEV detection by standard PCR

Veterinary Medicine inter-departmental subject scientific collection(110)
National Scientific Center "Institute of Experimental and Clinical Veterinary Medicine" (UA)
Openalex Percentile: Top 63%
Hepatitis Viruses Studies and Epidemiology
DOIOpen Access PDFOpenAlex
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Design of primer and linear probe sets for swine and wild boar HEV detection by PCR — O. Yu. Limanskaya · Veterinary Medicine inter-departmental subject scientific collection (2024) | TGRS Research Map | TGRS