Production Biotechnology for a Brucellosis Antigenic Latex Diagnosticum Based on Polyacrolein Microspheres

This research was aimed at developing a biotechnology for obtaining a latex brucellosis antigenic diagnosticum based on polyacrolein microspheres. This diagnosticum is intended for detecting antibodies against brucellosis in a latex agglutination reaction in human blood sera. Optimal conditions for latex sensitization with the antigen in the ratio of 1:3 at 45°C for 3 h followed by blocking of active centers with 0.5% gelatin were determined. The developed preparation showed high efficiency, which confirmed its prospects for brucellosis diagnostics. The analytical sensitivity of the diagnosticum was not less than 1:2560, in the absence of cross-reactions with heterologous sera. The diagnostic characteristics of experimental series of the preparation in control testing showed a sensitivity of 80% and a specificity of 93.33%.