Syzygium cumini fresh juice ameliorated memory impairment in a scopolamine-induced amnesic mouse model through active avoidance paradigm

The active avoidance paradigm is a reliable tool for the assessment of associative learning/memory. Nutritional manipulation is an emerging approach for the improvisation of brain health. Syzygium cumini (SC) fruit with enriched phytoconstituents, was investigated in varied concentrations on the learning/memory of scopolamine-induced amnesic mice through active-avoidance protocol. Chemical characterization by UHPLC-MS and molecular docking were performed for the identification of phytoconstituents and their action on possible targets. Our results revealed that SC-supplemented mice performed well as compared to amnesic group, with significant increase in the percentage avoidance and decrease in the latency. The identified phytoconstituents may enhance the cognitive performance in treated groups because of their antioxidant potential. Moreover, in-silico studies demonstrated good binding interactions with cholinesterase enzyme possibly inhibiting it and augmenting cholinergic activity in brain. In conclusion, SC dilutions improved cognitive performance in amnesic mice possibly due to antioxidant and anticholinesterase effects exerted by phyto-compounds. • Balanced nutritional approach is vital for brain health. • Syzygium cumini fruit juice was investigated in a scopolamine-induced amnesia. • Juice supplementation improved memory as validated by active avoidance test. • sphalleroside A, isobiflorin and dictyoquinazol were analyzed by docking studies. • Syzygium cumini significantly improved the cognitive performance possibly by antioxidant and anticholinergic mechanisms.