Efficiency of Penicillium commune to Biodegradation Crude oil

This study investigated the ability of Penicillium commune to degrade crude oil. This fungus was isolate from the soil contaminated with crude oil residues in the AL- Nasiriyah oil field (Al-Katea field) southern Iraq, using the dilution method, samples were collected quarterly, with one composite sample for each season, and the results were expressed quarterly, as the visits were divided into four visits and according to the number of seasons of the year. The results showed that the Penicillium commune was more frequent during this study, and the percentage appeared to reach 75% in soil contaminated with crude oil by diagnosed PCR technology, at different concentrations (1,2,3) %. The ability of Penicillium commune to degrade crude oil was studied in the presence of different carbon and nitrogen sources (glucose, maltose), (NaNo3, NH4Cl) and at different temperatures (18, 25, 42 oC) in the mineral salt’s medium. The results showed a decrease in the pH value, and the PH reached (3, 5,1) respectively after 28-day incubation, as well as the metabolic products and the remaining concentration that were calculated by gas chromatography -mass spectroscopy.