Phytochemical characterization and the therapeutic efficacy of Fraxinus americana

Fraxinus americana, a species commonly known as Ash tree is native to Eastern and Central North America and North Asia. This plant is known to posses a cluster of therapeutic properties. It is being used since ages in Unani and Ayurvedic medicine. Many infertility issues and gynecological problems can be treated by natural herbs and their extracts, Fraxinus is one such example. Objective of the current research is to characterize the various biochemical components present in the mother tincture of Fraxinus americana and testing its efficacy in treating uterine fibroids based on several biochemical and molecular assays. Phytochemical analysis based on biochemical study was performed followed by TLC analysis. TLC would provide an insight to the array of components present in tincture along with the information about the suitable solvents for their separation. MTT assay can be used to test the efficacy of the sample in controlling cell division (anti cancer). Use of uterine fibroid related cell lines (Human Uterine Leiomyoma cell culture) would identify its role in this condition. In addition to anti cancerous activity by MTT, various other tests like anti inflammatory and antioxidant activity would be performed to annotate the complete therapeutic potential of Fraxinus americana. The study can lay a foundation for a green therapy eliminating the need for surgical procedures in treating several uterine problems.