Monitoring and phylogenetic analysis of Cucumber mosaic virus isolates from Cucurbitaceae plants in Ukraine

Aim.To conduct monitoring and phylogenetic analysis of the cucumber mosaic virus isolates from agrocenoses of Ukraine.Methods.Enzyme-linked immunosorbent assay, RT-PCR, sequencing and phylogenetic analysis.Results.The agrocenoses of different regions of Ukraine were examined and the plants of Cucurbitaceae with the manifestations of viral diseases were selected.Using an enzymelinked immunosorbent assay, 131 samples of Cucurbitaceae plants were tested and the antigens of cucumber mosaic virus (CMV) were detected.The RT-PCR resulted in amplification products of 500 bp in size, which corresponds to the sequence of the capsid protein gene of CMV.Based on the sequence data, a phylogenetic tree of the selected CMV isolates was constructed.Conclusions.It was found that Ukrainian isolates of CMV obtained from the plants of Cucurbitaceae are represented only in the cluster of group I of subgroups IA and IB.The most related to the Ukrainian isolates were strains T35 and T19 (China), strain I17F (France) and banana (Israel).K e y w o r d s: Bromoviridae, cucumber mosaic virus (CMV), capsid protein gene, phylogenetic analysis.