A Rapid and Reliable Absorbance Assay to Identify Drug–Drug Interactions with Thiopurine Drugs
With purified TPMT on hand and the absorbance activity assay, we confirmed several compounds that inhibit TPMT, and the results were comparable to a mass spectral assay that measured 6-MP methylation. Understanding the impact of co-administered drugs on TPMT activity will improve the safety and efficacy of thiopurine-based treatment regimens.
Authors
- Rheem A. Totah (ORCID: https://orcid.org/0000-0003-1664-4874)
- Drake A. Russell (ORCID: https://orcid.org/0000-0001-9963-916X)
- Clarence E. Stafford
Institutions
- University of Washington (US)
Publication Details
- Journal
- Metabolites
- Published
- 2024-12-19
- DOI
- https://doi.org/10.3390/metabo14120715
- Citations
- 1
- Primary Topic
- Acute Lymphoblastic Leukemia research
- Type
- article
- Field-Weighted Citation Impact
- 0.54
Funders
- National Institutes of Health